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31.
Gerald W. Tannock Blair Lawley Karen Munro Ian M. Sims Julian Lee Christine A. Butts Nicole Roy 《Applied and environmental microbiology》2014,80(7):2240-2247
Knowledge of the trophisms that underpin bowel microbiota composition is required in order to understand its complex phylogeny and function. Stable-isotope (13C)-labeled inulin was added to the diet of rats on a single occasion in order to detect utilization of inulin-derived substrates by particular members of the cecal microbiota. Cecal digesta from Fibruline-inulin-fed rats was collected prior to (0 h) and at 6, 12, 18 and 24 h following provision of the [13C]inulin diet. RNA was extracted from these cecal specimens and fractionated in isopycnic buoyant density gradients in order to detect 13C-labeled nucleic acid originating in bacterial cells that had metabolized the labeled dietary constituent. RNA extracted from specimens collected after provision of the labeled diet was more dense than 0-h RNA. Sequencing of 16S rRNA genes amplified from cDNA obtained from these fractions showed that Bacteroides uniformis, Blautia glucerasea, Clostridium indolis, and Bifidobacterium animalis were the main users of the 13C-labeled substrate. Culture-based studies of strains of these bacterial species enabled trophisms associated with inulin and its hydrolysis products to be identified.
B. uniformis utilized Fibruline-inulin for growth, whereas the other species used fructo-oligosaccharide and monosaccharides. Thus, RNA–stable-isotope probing (RNA-SIP) provided new information about the use of carbon from inulin in microbiota metabolism. 相似文献
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Serrastruma lujae ants individually search for collembolans in the leaf litter of humid tropical forests. During the dry season, collembolans aggregate in wet patches randomly scattered in the dry litter where numerous single workers come hunting from their nest. We simulated this situation in the laboratory, and observed that workers seem to be able to use the humidity gradient direction to efficiently orient themselves towards a wet patch. Once the patch has been reached, they exhibit area-concentrated searching,consisting, in particular, of adopting a high sinuosity and a low speed. After capturing a collembolan, the ants return to their nest along nearly straight paths. This ability may rely on a spatial memory of the nest location by means of a path-integration process. In the absence of prey, however, various behaviours were observed after an unsuccessful search. Comparisons between these data and the results obtained with a homogeneously wet environment simulating the rainy season situation showed that these ants do not simply respond to the humidity level but are also sensitive to the degree of patchiness of their environment. They can therefore be said to be able to adapt suitably to the considerable climatic changes they encounter during the year. 相似文献
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This article is part of a Special Issue Energy Balance. 相似文献
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Nicole Poythress Waters Aaron M. StokerWilliam L. Carson Ferris M. PfeifferJames L. Cook 《Journal of biomechanics》2014
Osteoarthritis is one of the most common, debilitating, musculoskeletal diseases; 12% associated with traumatic injury resulting in post-traumatic osteoarthritis (PTOA). Our objective was to develop a single impact model with cartilage “injury level” defined in terms of controlled combinations of strain rate to a maximum strain (both independent of cartilage load resistance) to study their sensitivity to articular cartilage cell viability and potential PTOA biomarkers. A servo-hydraulic test machine was used to measure canine humeral head cartilage explant thickness under repeatable pressure, then subject it (except sham and controls) to a single impact having controlled constant velocity V=1 or 100 mm/s (strain rate 1.82 or 182/s) to maximum strain ε=10%, 30%, or 50%. Thereafter, explants were cultured in media for twelve days, with media changed at day 1, 2, 3, 6, 9, 12. Explant thickness was measured at day 0 (pre-injury), 6 and 12 (post-injury). Cell viability, and tissue collagen and glycosaminoglycan (GAG) were analyzed immediately post-injury and day 12. Culture media were tested for biomarkers: GAG, collagen II, chondroitin sulfate-846, nitric oxide, and prostaglandin E2 (PGE2). Detrimental effects on cell viability, and release of GAG and PGE2 to the media were primarily strain-dependent, (PGE2 being more prolonged and sensitive at lower strains). The cartilage injury model appears to be useful (possibly superior) for investigating the relationship between impact severity of injury and the onset of PTOA, specifically for discovery of biomarkers to evaluate the risk of developing clinical PTOA, and to compare effective treatments for arthritis prevention. 相似文献
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María B. Birkisdttir Dick Jaarsma Renata M. C. Brandt Sander Barnhoorn Nicole van Vliet Sandra Imholz Conny T. van Oostrom Bhawani Nagarajah Eliana Portilla Fernndez Anton J. M. Roks Ype Elgersma Harry van Steeg Jos A. Ferreira Jeroen L. A. Pennings Jan H. J. Hoeijmakers Wilbert P. Vermeij Martijn E. T. Doll 《Aging cell》2021,20(2)
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Dominique Martinez Antoine Chaffiol Nicole Voges Yuqiao Gu Sylvia Anton Jean-Pierre Rospars Philippe Lucas 《PloS one》2013,8(4)
Insects and robots searching for odour sources in turbulent plumes face the same problem: the random nature of mixing causes fluctuations and intermittency in perception. Pheromone-tracking male moths appear to deal with discontinuous flows of information by surging upwind, upon sensing a pheromone patch, and casting crosswind, upon losing the plume. Using a combination of neurophysiological recordings, computational modelling and experiments with a cyborg, we propose a neuronal mechanism that promotes a behavioural switch between surge and casting. We show how multiphasic On/Off pheromone-sensitive neurons may guide action selection based on signalling presence or loss of the pheromone. A Hodgkin-Huxley-type neuron model with a small-conductance calcium-activated potassium (SK) channel reproduces physiological On/Off responses. Using this model as a command neuron and the antennae of tethered moths as pheromone sensors, we demonstrate the efficiency of multiphasic patterning in driving a robotic searcher toward the source. Taken together, our results suggest that multiphasic On/Off responses may mediate olfactory navigation and that SK channels may account for these responses. 相似文献